Dates:
Oct 1st: For Students
Oct 3rd: For Industry and Academic Delegates

Objective of the workshop:

Molecular diagnostics and molecular research are experiencing a technology shift. qPCR (quantitative real-time PCR), a platform based on ultra sensitive detection of nucleic acids has made it possible to determine the amounts of specific nucleic acids and even proteins in biological samples with unsurpassed accuracy and sensitivity. This has opened new possibilities to study and diagnose diseases based on expression patterns and to detect pathogens in biological samples.

Level of the Course:
From beginners to intermediate

Course Outline

• Basic qPCR, applications and possibilities of the technology
• Available and forthcoming instrumentation
• The most common detection methods
• Primer design and PCR optimization
• Probe design for TaqMan and Molecular Beacons
• Design and performance of qPCR experiments.
• Pre-treatment of measured data
• Strategies for normalization
• Quantification strategies
• Reverse transcription
• Extraction of DNA and RNA
• Quality assessment in qPCR
• SNP and mutation analysis using qPCR
• Multiplex qPCR
• Immuno qPCR for protein detection
• Multimarker qPCR diagnostics
• Digital PCR
• Future developments in qPCR and novel applications

Michael was among the pioneers who developed real-time PCR. Starting in 1991 his laboratory developed dyes and probes for real-time PCR and founded LightUp Technologies as the first company in the world focusing on real-time PCR based human infectious disease testing. His team then developed experimental approaches for accurate measurements of expression levels, and they pioneered the fields of single cell expression profiling and multidimensional expression profiling by real-time PCR. Kubista also developed methods and approaches to analyze gene expression data and co-founded MultiD Analyses that develops the popular software GenEx for processing and analyses of real-time PCR data. Working as advisor for Unesco he introduced real-time PCR in Africa and in the Middle East. In 2001 Kubista founded the TATAA Biocenters as the world leading service providers and organizers of hands-on training in real-time PCR. Regular training courses are held all over Europe, Africa, Asia and in the US. The TATAA courses world-wide are supported by leading instrument manufacturers and reagents suppliers in the real-time PCR field. Kubista is also advisor to several biotech and pharmaceutical companies.

About TATAA Biocenter. TATAA Biocenter with locations in Gothenburg, Sweden, Prague, Czech Republic, Freising, Germany, and Sunnyvale, CA, is leading global provider of real-time PCR services. TATAA Biocenter organizes hands-on training in real-time PCR, and conducts research and development in diagnostics and gene expression profiling based on real-time PCR for industry and the public sector. For course schedule and further information about TATAA Biocenter, see www.tataa.com.

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