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Automation of capture sequence and probe design |
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Customer:
- US Based Nano-biotech company
Key Customer Challenges:
- Customer is a developer of Spot Assay technology for colorimetric detection of amplified DNA sequences using gold nanoparticle probe technology. The nanoparticle probes change color based on the presence of a specific DNA target and function as permanent test record when spotted onto a membrane.
- Customer required a custom tool to design capture sequences for unique regions and probe sequences for common regions between the input sequences for use with their technology.
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Solution Overview
- Custom Bioinformatics Application developed for requirement
- The application was developed to accept an input of at least two or more sequences (CDS) of a single gene or gene sequences from different organisms or species
- Application generated Capture sequences in a complementary fashion to certain unique regions on each of the input sequence like SNPs
- Application generated Probe sequences that were down stream to the capture sequences
- For each input sequence, the capture sequences and the probes would be generated and displayed along with their properties
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Key Benefits to Customer
- Automated a highly manual process
- Intelligent algorithms to ensure quality and consistency
- Platform was rendered customizable due to inclusion of software and ability to accept user inputs
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