• Polyclone developed ‘eprime’ a first of its kind software in the Real-time PCR market
• eprime is a stand-alone tool for designing primers and probes for qPCR assays
• It offers separate modules for SYBR® Green, TaqMan® and Molecular Beacons as well as for primer design for conventional PCR and restriction mapping, along with high throughput option, making it a unique system
• Packaged as a license to customers of Realtime PCR equipment

• Accuracy: Engineered for effective qPCR assay design, with focus on assay efficiency and sensitivity and minimal time for optimization. It scans the complete input sequence with speed and accuracy to output stringent and specific oligos.
• Ease, Speed and Customizability: User-friendly, automated batch input, accurate, maintenance maintenance free and low cost tool for effective design of primers and probes with adaptability for user defined parameters.
• Auto-correction: Besides reporting the complete oligo information on successful design, it also provides the salient redesign option for failed templates
• Modular: The modular oligo design for conventional PCR, SYBR® Green, TaqMan® gene expression, Taqman® SNP and Molecular Beacon provides easy accessibility to specific section along with the defined parameters.

The customer was able to facilitate the optimal usage of their equipment by their customers, thus increasing customer-satisfaction and retention. The following were the benefits to end customers:

• High Throughput: Batch input template submission  as excel or text file for high throughput applications
• Complete Analysis: Direct link for BLAST and secondary structure analysis make it a one stop solution. Input private files or edit existing sequences as input
• Visual Depiction: Export the results to excel sheet or pdf, a colorful graphical interface displayed on the screen provides an overall picture of the designed primers
• Independent: eprime also provides the flexibility of a stand alone application to derive Tm, blast results and secondary structures for the designed primers for any nucleotide sequence