Cell and Stemcell Based Services for Benchmarking and Drug Discovery Studies           Polyclone offers comprehensive cell biology services for target and lead validation research, mechanism of action studies and benchmarking studies through primary cell & tissue culture, cell based screening assays and stable cell line development. Across these areas, Polyclone adds value by addressing specific assay requirements to enable customers achieve their research goals. Our cell biology services also complement our molecular biology services to ensure complete research solutions. In extension, we also offer complementary services ranging from immunocytochemistry to cell sorting.           Custom cell culture and cellline maintenance Cell sorting and culture services for all types of cells including elusive categories with hands-on experience in isolation and growth of several primary cell cultures such as: Adipocyte cultures B-cells Breast cancer stem cells Buccal mucosa epithelial cells Chondrocytes Cord blood stem cells Epithelial cells Hepatocytes Keratinocytes Limbal stem cells Melanoma cancer cells Mesenchymal stem cells Monocytes and macrophages Neuron cell cultures Osteoblasts Osteoclasts T-cells Wharton's jelly cells Maintenance, expansion, transfection and stable cell line generation services for target validation: Cell cloning & selection Cell Expansion Protein expression ----------------------------------------------------------------------------- Top -------------------------------------------------------------------------------   Cell based assays Polyclone's cell biology team can perform various cell-based assays using cell lines and in-house generated primary cultures for understanding mode of action of drug candidates at cellular level. Our expertise spans across various cell-based assays, optimization of culture medium and high-throughput screening assays. Proliferation/Apoptosis assays Transmigration assays Cell Invasion assays Phagocytosis assays Angiogenesis assays Wound healing assays Metabolic & functional assays Cytotoxicity assays Signal transduction assays Tumour sensitivity assays Cell adhesion assays Chemotaxis assays ----------------------------------------------------------------------------- Top -------------------------------------------------------------------------------   Stemcell based therapy Polyclone offers stemcell isolation and expansion services for stemcell banking and stemcell based therapy studies. Stemcells are isolated from various sources and differentiated into respective progenitor cells and cell lineages.     Stemcell expansion services for various regenerative medicine therapies are also offered. This includes processing, extraction and expansion of stemcells from various sources for limbal therapy, stemcell therapy for liver cirrhosis and lower limb ischemia among others. ----------------------------------------------------------------------------- Top -------------------------------------------------------------------------------   Immunocytochemistry Services Expertise in immunocytochemistry ranges from flow cytometry to confocal imaging on cells and tissues. Flow Cytometry Propidium iodide and 7AAD Immunophenotyping Cytokine expression and cell signaling Confocal Imaging Surface marker expression Co-localization studies Live cell imaging ----------------------------------------------------------------------------- Top -------------------------------------------------------------------------------   Cell Sorting Magnetic based cell sorting ranging from research grade to clinical grade Magnetic based cell sorting Flow cytometry based cell sorting ----------------------------------------------------------------------------- Top -------------------------------------------------------------------------------

Custom cell culture and cellline maintenance     Cell based assays     Stemcell based therapy     Immunocytochemistry Services     Cell Sorting

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